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CNTF, human recombinant protein
HCNTF, CNTF, Ciliary Neurotrophic Factor.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P26441 |
|---|---|
| Calculated MW | 22.9 kDa |
| Gene ID | 1270 |
|---|---|
| Gene Symbol | CNTF |
| Other Names | HCNTF, CNTF, Ciliary Neurotrophic Factor. |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | HPLC; ≥98% |
| Recombinant | Yes |
| Results | < 2 ng/ml |
| Target/Specificity | CNTF |
| Application Notes | Reconstitute in 5 mM Sodium Phosphate, pH 7.5 to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use |
| Format | Lyophilized protein |
| Storage | -20°C; Sterile filtered and lyophilized without additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
CNTF (Ciliary Neurotrophic Factor) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF has also been shown to promote survivability and differentiation of other neuronal cell types. Recombinant human CNTF is a 22.9 kDa protein containing 200 amino acid residues
References
Masiakowski P.,et al.J. Neurochem. 57:1003-1012(1991).
Negro A.,et al.Eur. J. Biochem. 201:289-294(1991).
Lam A.,et al.Gene 102:271-276(1991).
McDonald J.R.,et al.Biochim. Biophys. Acta 1090:70-80(1991).
Takahashi R.,et al.Nat. Genet. 7:79-84(1994).
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