GM-CSF, human recombinant protein
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-α
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P04141 |
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Calculated MW | 14.6 kDa |
Gene ID | 1437 |
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Gene Symbol | CSF2 |
Other Names | Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-α, Colony-stimulating factor, Molgramostin, Sargramostim |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | <0.1 ng/ml, |
Target/Specificity | GM-CSF |
Application Notes | Reconstitute in H₂O to a concentration of 0.1-1.0 µg/ µl. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from 10 mM Sodium Citrate, pH 3.5 |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human GM-CSF (Granulocyte Macrophage Colony stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein containing 128 amino acid residues.
References
Lee F.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:4360-4364(1985).
Kaushansky K.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:3101-3105(1986).
Cantrell M.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6250-6254(1985).
Wong G.G.,et al.Science 228:810-815(1985).
Miyatake S.,et al.EMBO J. 4:2561-2568(1985).
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