IFN-gamma, murine recombinant protein
Immune Interferon-γ, type II interferon, T cell interferon, MAF
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P01580 |
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Calculated MW | 15.6 kDa |
Gene ID | 15978 |
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Gene Symbol | IFNG |
Other Names | Immune Interferon-γ, type II interferon, T cell interferon, MAF |
Gene Source | Mouse |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; ≥95% |
Recombinant | Yes |
Results | <0.1 ng/ml |
Target/Specificity | IFN-gamma |
Application Notes | Reconstitute in ddH₂O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from 10 mM Na2PO4, pH 7.0 + 25 mM NaCl |
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Provided below are standard protocols that you may find useful for product applications.
Background
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Recombinant murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues.
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