IL-10, murine recombinant protein
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P18893 |
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Calculated MW | 18.7 kDa |
Gene ID | 16153 |
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Gene Symbol | IL10 |
Other Names | B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. |
Gene Source | Mouse |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | <2 ng/ml |
Target/Specificity | IL-10 |
Application Notes | Reconstitute in 3 mM Tris, pH 8.0 to a concentration of 0.1-1.0 µg/ µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from 3 mM Tris, pH 8.0 |
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Provided below are standard protocols that you may find useful for product applications.
Background
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-Ą. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant murine IL-10 is an 18.7 kDa protein of 161 amino acid residues.
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