ProNGF, human recombinant protein
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P01138 |
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Calculated MW | ~25 kDA |
Gene ID | 4803 |
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Gene Symbol | NGF |
Other Names | Beta-nerve growth factor (Beta-NGF) |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; ≥95% |
Recombinant | Yes |
Sequence | Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser. |
Target/Specificity | ProNGF |
Application Notes | Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 µg/ml.). This can further be diluted to other aqueous buffers. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from a 0.2 µm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystine knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
References
Ullrich A.,et al.Nature 303:821-825(1983).
Ullrich A.,et al.Cold Spring Harb. Symp. Quant. Biol. 48:435-442(1983).
Borsani G.,et al.Nucleic Acids Res. 18:4020-4020(1990).
Tong Y.,et al.Zhongguo Ying Yong Sheng Li Xue Za Zhi 13:316-318(1997).
Kitano T.,et al.Mol. Biol. Evol. 21:936-944(2004).
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