Apo-SAA, human recombinant protein
Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P0DJI8 |
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Calculated MW | 11.5 kDa |
Gene ID | 6288 |
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Gene Symbol | SAA1 |
Other Names | Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, Apolipoprotein, apolipoproteins, SAA1, SAA2, PIG4, TP53I4, MGC111216, |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | 10-50 µg/ml |
Target/Specificity | Apo-SAA |
Application Notes | Reconstitute in 0.1% acetic acid to a concentration of 1 µg/ µl. This solution can then be diluted into other aqueous buffers. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5 µg/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence.
References
Sipe J.D.,et al.Biochemistry 24:2931-2936(1985).
Kluve-Beckerman B.,et al.J. Clin. Invest. 82:1670-1675(1988).
Betts J.,et al.Scand. J. Immunol. 34:471-482(1991).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Taylor T.D.,et al.Nature 440:497-500(2006).
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