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SF20, mouse recombinant protein
UPF0556 protein C19orf10 homolog, Stromal cell-derived growth factor SF20, Interleukin-25, IL-25, D1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9CPT4 |
|---|---|
| Calculated MW | 15.8 kDa |
| Gene ID | 28106 |
|---|---|
| Gene Symbol | CS010 |
| Other Names | UPF0556 protein C19orf10 homolog, Stromal cell-derived growth factor SF20, Interleukin-25, IL-25, D17Wsu104e, Il25, SF20, R33729_1, C19orf10, Chromosome 19 Open Reading Frame 10. |
| Gene Source | Mouse |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥97% |
| Assay2&Purity2 | HPLC; ≥97% |
| Recombinant | Yes |
| Sequence | MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL |
| Target/Specificity | SF20 |
| Application Notes | Reconstitute in sterile ddH₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers. |
| Format | Lyophilized protein |
| Storage | -20°C; Lyophilized from a sterile filtered solution containing PBS pH 7.4 |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse SF20 is a bone marrow stroma-derived growth factor. SF20 is expressed in the bone marrow, spleen stroma cells, resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells. SF20 has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Among SF20’s biological activities is stimulation of the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells. Chromosome 19 Open Reading Frame 10 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 15.8kDa. The Mouse SF20 is purified by proprietary chromatographic techniques.
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