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Leptin, rat recombinant protein
OB Protein, Obesity Protein, OBS, Obesity factor.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P50596 |
|---|---|
| Calculated MW | 16.2 kDa |
| Gene ID | 25608 |
|---|---|
| Gene Symbol | LEP |
| Other Names | OB Protein, Obesity Protein, OBS, Obesity factor. |
| Gene Source | Rat |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; ≥95% |
| Recombinant | Yes |
| Results | 0.35-0.06 ng/ml |
| Target/Specificity | Leptin |
| Application Notes | Reconstitute in H₂O to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA). |
| Format | Lyophilized protein |
| Storage | -20°C; Lyophilized from 4.5 µM NaHCO₃. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.
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