SDF-1beta/CXCL12, feline recombinant protein
SDF1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O62657 |
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Calculated MW | 8.5 kDa |
Gene ID | 493806 |
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Gene Symbol | SDF1 |
Other Names | C-X-C motif chemokine 12, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor |
Gene Source | Feline |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥97% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Results | 10 - 75 ng/ml. |
Sequence | KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRF KM |
Target/Specificity | SDF-1 beta |
Application Notes | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Stromal Derived Growth Factor-1beta (SDF-1beta), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1β signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human SDF-1β shares 100% identity to feline SDF-1β. Recombinant feline SDF-1beta is a non-glycosylated protein, comprised of 72 amino acids, with a molecular weight of 8.5 kDa.
References
Nishimura Y.,et al.Eur. J. Immunogenet. 25:303-305(1998).
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