Growth Hormone, chicken recombinant protein
Growth hormone 1, Somatotropin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P08998 |
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Calculated MW | 22.255 kDa |
Gene ID | 378781 |
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Gene Symbol | SOMA |
Other Names | Growth hormone 1, Somatotropin, Pituitary growth hormone |
Gene Source | Chicken |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥99% |
Assay2&Purity2 | HPLC; ≥99% |
Recombinant | Yes |
Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile H₂O or 0.4 % NaHCO₃ adjusted to pH 8-9 to a concentration ≥ 100 µg/ml and sterile filtered. This solution can then be diluted into other aqueous buffers, preferably in the presence of carrier protein. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from 0.3 % NaHCO₃ , pH 8 |
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Provided below are standard protocols that you may find useful for product applications.
Background
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Growth Hormone Chicken Recombinant (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. GH Chicken recombinant is purified by proprietary chromatographic techniques.
References
Lamb I.C.,et al.Nucleic Acids Res. 16:9339-9339(1988).
Zhvirblis G.S.,et al.Mol. Biol. (Mosk.) 21:1324-1328(1987).
Zhvirblis G.S.,et al.Mol. Biol. (Mosk.) 21:1620-1624(1987).
Baum D.,et al.Comp. Biochem. Physiol. 96B:491-495(1990).
Tanaka M.,et al.Gene 112:235-239(1992).
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