Active HIV1 Protease recombinant protein (GST-tagged)
HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9YQ30 |
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Concentration | 3 |
Calculated MW | 38.4 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31 kDa during SEC and SDS-PAGE analyses. |
Gene Symbol | HIV1 protease |
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Other Names | HIV-1 retropepsin, HIV-1 Protease (PR), cd05482 |
Gene Source | HIV1 |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | 1-99 aa + N-terminal GST and C-terminal Poly-his tags |
Target/Specificity | HIV1 Protease |
Format | Liquid |
Storage | -80°C; 3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells.
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