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ALDH3B1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ALDH3B1 Antibody (Center) AP8706C
    All lanes : Anti-ALDH3B1 Antibody (Center) at 1:1000 dilution Lane 1: human kidney lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - ALDH3B1 Antibody (Center) AP8706C
    Western blot analysis of ALDH3B1 Antibody (Center) (Cat. #AP8706c) in MDA-MB231 cell line lysates (35ug/lane). ALDH3B1 (arrow) was detected using the purified Pab.
    detail
  • WB - ALDH3B1 Antibody (Center) AP8706C
    Western blot analysis of ALDH3B1 (arrow) using rabbit polyclonal ALDH3B1 Antibody (Center) (Cat. #AP8706c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH3B1 gene.
    detail
  • IHC-P - ALDH3B1 Antibody (Center) AP8706C
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ALDH3B1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P43353
Other Accession Q5XI42, E9Q3E1
Reactivity Human
Predicted Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51840 Da
Antigen Region 334-360 aa
Additional Information
Gene ID 221
Other Names Aldehyde dehydrogenase family 3 member B1, Aldehyde dehydrogenase 7, ALDH3B1, ALDH7
Target/Specificity This ALDH3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-360 amino acids from the Central region of human ALDH3B1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH3B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH3B1
Synonyms ALDH7
Function Oxidizes medium and long chain saturated and unsaturated aldehydes (PubMed:17382292, PubMed:23721920). Metabolizes also benzaldehyde (PubMed:17382292). Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde (PubMed:17382292, PubMed:23721920). May not metabolize short chain aldehydes. Can use both NADP(+) and NAD(+) as electron acceptor (PubMed:17382292). May have a protective role against the cytotoxicity induced by lipid peroxidation (PubMed:17382292).
Cellular Location Cell membrane; Lipid-anchor. Note=Primarily in the plasma membrane as well as in some punctate structures in the cytoplasm
Tissue Location Highest expression in kidney and lung.
Research Areas
Citations ( 0 )

Background

The aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.

References

Marchitti,S.A., et.al., Biochem. Biophys. Res. Commun. 356 (3), 792-798 (2007)

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$ 365.00
$ 140.00
Cat# AP8706C
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