SLC22A6 Antibody (C-Term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | Q4U2R8 |
Other Accession | NP_004781 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 61816 Da |
Antigen Region | 513-541 aa |
Gene ID | 9356 |
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Other Names | Solute carrier family 22 member 6, Organic anion transporter 1, hOAT1, PAH transporter, hPAHT, Renal organic anion transporter 1, hROAT1, SLC22A6, OAT1, PAHT |
Target/Specificity | This SLC22A6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the C-terminal region of human SLC22A6. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SLC22A6 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SLC22A6 (HGNC:10970) |
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Synonyms | OAT1, PAHT |
Function | Secondary active transporter that functions as a Na(+)- independent organic anion (OA)/dicarboxylate antiporter where the uptake of one molecule of OA into the cell is coupled with an efflux of one molecule of intracellular dicarboxylate such as 2-oxoglutarate or glutarate (PubMed:9950961, PubMed:11907186, PubMed:11669456, PubMed:14675047, PubMed:22108572, PubMed:23832370, PubMed:28534121). Mediates the uptake of OA across the basolateral side of proximal tubule epithelial cells, thereby contributing to the renal elimination of endogenous OA from the systemic circulation into the urine (PubMed:9887087). Functions as a biopterin transporters involved in the uptake and the secretion of coenzymes tetrahydrobiopterin (BH4), dihydrobiopterin (BH2) and sepiapterin to urine, thereby determining baseline levels of blood biopterins (PubMed:28534121). Transports prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) and may contribute to their renal excretion (PubMed:11907186). Also mediates the uptake of cyclic nucleotides such as cAMP and cGMP (PubMed:26377792). Involved in the transport of neuroactive tryptophan metabolites kynurenate (KYNA) and xanthurenate (XA) and may contribute to their secretion from the brain (PubMed:22108572, PubMed:23832370). May transport glutamate (PubMed:26377792). Also involved in the disposition of uremic toxins and potentially toxic xenobiotics by the renal organic anion secretory pathway, helping reduce their undesired toxicological effects on the body (PubMed:11669456, PubMed:14675047). Uremic toxins include the indoxyl sulfate (IS), hippurate/N- benzoylglycine (HA), indole acetate (IA), 3-carboxy-4- methyl-5-propyl- 2-furanpropionate (CMPF) and urate (PubMed:14675047, PubMed:26377792). Xenobiotics include the mycotoxin ochratoxin (OTA) (PubMed:11669456). May also contribute to the transport of organic compounds in testes across the blood-testis-barrier (PubMed:35307651). |
Cellular Location | Basolateral cell membrane; Multi-pass membrane protein. Basal cell membrane; Multi-pass membrane protein. Note=Localized to the basolateral membrane of renal proximal tubular cells (PubMed:9887087) Localized to the basal membrane of Sertoli cells (PubMed:35307651) |
Tissue Location | Strongly expressed in kidney (PubMed:9887087, PubMed:9950961, PubMed:10049739, PubMed:10462545, PubMed:10964714) Expressed at lower level in liver, skeletal muscle, brain and placenta (PubMed:9887087, PubMed:9950961, PubMed:10049739, PubMed:10462545). In kidney, found at the basolateral membrane of the proximal tubule (PubMed:9887087). In testis, primarily localized to the basal membrane of Sertoli cells and weakly expressed in Leydig cells and vascular endothelial cells (PubMed:35307651). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein is involved in the sodium-dependent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and may be localized to the basolateral membrane.
References
Hong,M., et.al, J. Pharmacol. Exp. Ther. 332 (2), 650-658 (2010)
Shin,H.J., et.al, Clin. Chim. Acta 411 (1-2), 99-105 (2010)
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