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DANRE arnt2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - DANRE arnt2 Antibody (Center) Azb10020a
    DANRE arnt2 Antibody (Center) (Cat. #Azb10020a) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9DG12
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 475-501 aa
Additional Information
Other Names Aryl hydrocarbon receptor nuclear translocator 2, ARNT protein 2, zfARNT2, arnt2 {ECO:0000312|ZFIN:ZDB-GENE-001207-3}
Target/Specificity This DANRE arnt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-501 amino acids from the Central region of DANRE arnt2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE arnt2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name arnt2 {ECO:0000312|ZFIN:ZDB-GENE-001207-3}
Function Transcription factor that plays a role in the development of the hypothalamo-pituitary axis. Specifically recognizes the xenobiotic response element (XRE) (By similarity). Isoform 1 acts as a transcriptional activator (PubMed:11072074). Isoform 3 acts as a transcriptional repressor (PubMed:10960127, PubMed:11072074).
Cellular Location Nucleus.
Tissue Location Isoform 1 and isoform 2 are most highly expressed in the brain, eye and skeletal muscle and to a lower degree in liver, heart, kidney and swim bladder. Isoform 3 is most highly expressed in the eye, forebrain, midbrain, hindbrain, skeletal muscle, gills and brain but is barely detectable in liver, heart, kidney and swim bladder. Before the pharyngula period isoform 3 is expressed throughout the entire embryo and during this period extensively in the brain and eye. EMBL; AF219987; AAG25919.1; -; mRNA EMBL; AF219988; AAG25920.1; -; mRNA EMBL; AF219989; AAG25921.1; -; mRNA EMBL; AF155066; AAF73280.1; -; mRNA RefSeq; NP_571749.1; NM_131674.1 SMR; Q9DG12; - STRING; 7955.ENSDARP00000110276; - PaxDb; Q9DG12; - PRIDE; Q9DG12; - GeneID; 64277; - KEGG; dre:64277; - CTD; 9915; - ZFIN; ZDB-GENE-001207-3; arnt2 eggNOG; KOG3561; Eukaryota InParanoid; Q9DG12; - KO; K15589; - PhylomeDB; Q9DG12; - PRO; PR:Q9DG12; - Proteomes; UP000000437; Unplaced GO; GO:0005737; C:cytoplasm; IEA:InterPro GO; GO:0005634; C:nucleus; IC:UniProtKB GO; GO:0005667; C:transcription regulator complex; IEA:InterPro GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB GO; GO:0046982; F:protein heterodimerization activity; NAS:UniProtKB GO; GO:0007411; P:axon guidance; IMP:ZFIN GO; GO:0007420; P:brain development; ISS:UniProtKB GO; GO:0003208; P:cardiac ventricle morphogenesis; IMP:ZFIN GO; GO:0098609; P:cell-cell adhesion; IMP:ZFIN GO; GO:0021536; P:diencephalon development; IMP:ZFIN GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:ZFIN GO; GO:0021979; P:hypothalamus cell differentiation; IMP:ZFIN GO; GO:0072576; P:liver morphogenesis; IMP:ZFIN GO; GO:0007275; P:multicellular organism development; NAS:UniProtKB GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IPI:ZFIN GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB GO; GO:0001666; P:response to hypoxia; IDA:UniProtKB GO; GO:0007165; P:signal transduction; IDA:UniProtKB GO; GO:0048798; P:swim bladder inflation; IMP:ZFIN GO; GO:0021591; P:ventricular system development; IMP:ZFIN CDD; cd00083; HLH; 1 CDD; cd00130; PAS; 2 Gene3D;; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf InterPro; IPR001067; Nuc_translocat InterPro; IPR001610; PAC InterPro; IPR000014; PAS InterPro; IPR035965; PAS-like_dom_sf InterPro; IPR013767; PAS_fold InterPro; IPR013655; PAS_fold_3 Pfam; PF00010; HLH; 1 Pfam; PF00989; PAS; 1 Pfam; PF08447; PAS_3; 1 PRINTS; PR00785; NCTRNSLOCATR SMART; SM00353; HLH; 1 SMART; SM00086; PAC; 1 SMART; SM00091; PAS; 2 SUPFAM; SSF47459; SSF47459; 1 SUPFAM; SSF55785; SSF55785; 2 TIGRFAMs; TIGR00229; sensory_box; 1 PROSITE; PS50888; BHLH; 1 PROSITE; PS50112; PAS; 2 1: Evidence at protein level; Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation CHAIN 1..737 /note="Aryl hydrocarbon receptor nuclear translocator 2" /id="PRO_0000239938" DOMAIN 78..131 /note="bHLH" /evidence="ECO:0000255|PROSITE-ProRule:PRU00981" DOMAIN 149..221 /note="PAS 1" /evidence="ECO:0000255|PROSITE-ProRule:PRU00140" DOMAIN 340..406 /note="PAS 2" /evidence="ECO:0000255|PROSITE-ProRule:PRU00140" DOMAIN 413..456 /note="PAC" /evidence="ECO:0000255" COMPBIAS 455..461 /note="Poly-Gln" /evidence="ECO:0000255" COMPBIAS 529..665 /note="Ser-rich" /evidence="ECO:0000255" VAR_SEQ 66..80 /note="Missing (in isoform 2)" /evidence="ECO:0000303|PubMed:11072074" /id="VSP_052058" VAR_SEQ 405..425 /note="VKLKGQVLSVMYRFRMKNREW -> RQNQIKPVSQHQSHISHMMFF (in isoform 3)" /evidence="ECO:0000303|PubMed:10960127, ECO:0000303|PubMed:11072074" /id="VSP_052059" VAR_SEQ 426..737 /note="Missing (in isoform 3)" /evidence="ECO:0000303|PubMed:10960127, ECO:0000303|PubMed:11072074" /id="VSP_052060" CONFLICT 137 /note="T -> A (in Ref. 1; AAG25920)" /evidence="ECO:0000305" CONFLICT 370 /note="I -> T (in Ref. 1; AAG25920)" /evidence="ECO:0000305" CONFLICT 379 /note="D -> V (in Ref. 2; AAF73280)" /evidence="ECO:0000305" CONFLICT 438 /note="P -> S (in Ref. 1; AAG25921)" /evidence="ECO:0000305" CONFLICT 495 /note="T -> S (in Ref. 1; AAG25921)" /evidence="ECO:0000305" CONFLICT 625 /note="A -> V (in Ref. 1; AAG25921)" /evidence="ECO:0000305" SEQUENCE 737 AA; 80972 MW; 96CB457F52B12F09 CRC64; MATPAAVNPS EMGTDLPGPV SMPGAVVGAG QVRMTGAMPG RGGKRRSAGM DFDDEDGEGP SKFSRYDDDQ IPGDKERYAR ENHSEIERRR RNKMTQYITE LSDMVPTCSA LARKPDKLTI LRMAVSHMKS MRGTGNTSTD GAYKPSFLTE QELKHLILEA ADGFLFVVAA ETGRVIYVSD SVTPVLNHPQ SEWFGSTLFE QVHPDDVDKL REQLSTSENS MTGRILDLKT GTVKKEGQQS SMRMCMGSRR SFICRMRCGS APLDHISLNR LSSMRKRYRN GLGPSKEGEA QYSVVHCTGY IKAWPPAGMT IPDEDTEAGQ TSKYCLVAIG RLQVTSSPVS MDMNGLSVPT EFLSRHNSDG IITFVDPRCI NVIGYQPQDL LGKDILEFCH PEDQSHLRES FQQVVKLKGQ VLSVMYRFRM KNREWMLIRT SSFTFQNPYS DEIEYIICTN TNVKQLQQQQ QAELEVHQRD GLTAYDLSQV PVSGVSAGVH ESGKTIDKTE SLFSQERDPR FSDIYTGIST SEKKMMVPSS TSGGQQLYSQ GSPFQPGHSG KSFSSSVIHV PGVNDIQSTA GSAGQNLSQI SRQINTGQVS WSGNRPPFSG QQIPAQSNKA QSSPFGIGSS HSYQADPSSY SPLSSPATSS PSGNAYSNLA NRNTAFDVSG ESSQSGGQFQ GRPSEVWSQW QSQHHSQQAG DPHPHTQASQ TEVFQDMLPM PGDPTQGTTN YNIEDFADLG MFPPFSE
Research Areas
Citations (0)

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Specifically recognizes the xenobiotic response element (XRE). Isoform 1 acts as a transcriptional activator. Isoform 3 acts as a transcriptional repressor.


Tanguay R.L., et al. Biochim. Biophys. Acta 1494:117-128(2000).
Wang W.-D., et al. Mar. Biotechnol. 2:376-386(2000).

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Cat# Azb10020a
Availability: Back Order
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