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DANRE shha Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q92008 |
| Reactivity | Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone/Animal Names | RB46723 |
| Calculated MW | 46403 Da |
| Antigen Region | 151-162 aa |
| Gene ID | 30269 |
|---|---|
| Other Names | Sonic hedgehog protein A, SHHA, VHH-1, Sonic hedgehog protein A N-product, Sonic hedgehog protein A C-product, shha, shh, vhh1 |
| Target/Specificity | This shha antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 151-162 amino acids from the Central region of DANRE shha. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DANRE shha Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | shha |
|---|---|
| Synonyms | shh, vhh1 |
| Function | [Sonic hedgehog protein]: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). |
| Cellular Location | [Sonic hedgehog protein]: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q15465}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q15465}. Note=Co-localizes with HHAT in the ER and Golgi membrane. {ECO:0000250|UniProtKB:Q15465} |
| Tissue Location | Expressed in the ventral midline of the neural tube and brain. Also found in the notochord and in developing fin bud. In the developing brain, expression occurs in domains that include a discrete region in the floor of the diencephalon |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning, dorso-ventral patterning of the brain and early patterning of the developing eyes. Displays floor plate-inducing activity. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO (By similarity).
References
Roelink H.,et al.Cell 76:761-775(1994).
Ekker S.C.,et al.Curr. Biol. 5:944-955(1995).
Fietz M.J.,et al.Development Suppl. 120:43-51(1994).
Muller F.,et al.Development 126:2103-2116(1999).
Zardoya R.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:13036-13041(1996).
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