|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||25097 Da|
|Other Names||Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F|
|Target/Specificity||The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site.|
|Format||affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-EIF4E-S209 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.|
|Cellular Location||Cytoplasm, P-body. Cytoplasm|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).
Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983.
The virion host shutoff endonuclease (UL41) of herpes simplex virus interacts with the cellular cap-binding complex eIF4F. Page HG, et al. J Virol, 2010 Jul. PMID 20427534.
HDAC2 promotes eIF4E sumoylation and activates mRNA translation gene specifically. Xu X, et al. J Biol Chem, 2010 Jun 11. PMID 20421305.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Interaction of Pdcd4 with eIF4E inhibits the metastatic potential of hepatocellular carcinoma. Jiang Y, et al. Biomed Pharmacother, 2010 Jul. PMID 20359850.
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