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Phospho-EIF4E-S209 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P06730 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Concentration | 1mg/ml |
| Isotype | Rabbit IgG |
| Calculated MW | 25097 Da |
| Gene ID | 1977 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F |
| Target/Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Dilution | WB~~1:500~1:1000 IHC~~1:50~1:100 |
| Format | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-EIF4E-S209 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EIF4E (HGNC:3287) |
|---|---|
| Synonyms | EIF4EL1, EIF4F |
| Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:16271312, PubMed:22578813). In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm (PubMed:24335285). Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap (By similarity). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity). In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285). May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity). |
| Cellular Location | Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus Note=Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257) |
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Provided below are standard protocols that you may find useful for product applications.
Background
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).
References
Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983.
The virion host shutoff endonuclease (UL41) of herpes simplex virus interacts with the cellular cap-binding complex eIF4F. Page HG, et al. J Virol, 2010 Jul. PMID 20427534.
HDAC2 promotes eIF4E sumoylation and activates mRNA translation gene specifically. Xu X, et al. J Biol Chem, 2010 Jun 11. PMID 20421305.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Interaction of Pdcd4 with eIF4E inhibits the metastatic potential of hepatocellular carcinoma. Jiang Y, et al. Biomed Pharmacother, 2010 Jul. PMID 20359850.
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