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HLA-A Antibody(Center)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P04439 |
| Other Accession | P16188, P13746 |
| Reactivity | Mouse |
| Predicted | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,Ig |
| Clone/Animal Names | 7G7F9 |
| Calculated MW | 40841 Da |
| Antigen Region | 70-99 aa |
| Gene ID | 3105 |
|---|---|
| Other Names | HLA class I histocompatibility antigen, A-3 alpha chain, MHC class I antigen A*3, HLA-A, HLAA |
| Target/Specificity | This HLA-A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the Central region of human HLA-A. |
| Dilution | WB~~1:500~1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HLA-A Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HLA-A (HGNC:4931) |
|---|---|
| Synonyms | HLAA |
| Function | Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells (PubMed:2456340, PubMed:2784196, PubMed:1402688, PubMed:7504010, PubMed:9862734, PubMed:10449296, PubMed:12138174, PubMed:12393434, PubMed:15893615, PubMed:17189421, PubMed:19543285, PubMed:21498667, PubMed:24192765, PubMed:7694806, PubMed:24395804, PubMed:28250417). May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity (PubMed:25880248, PubMed:7506728, PubMed:7679507). Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (PubMed:12796775, PubMed:18275829, PubMed:19542454, PubMed:28250417). Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme (PubMed:17189421, PubMed:20364150, PubMed:17079320, PubMed:26929325, PubMed:27049119). Can bind different peptides containing allele- specific binding motifs, which are mainly defined by anchor residues at position 2 and 9 (PubMed:7504010, PubMed:9862734). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Tissue Location | Ubiquitous.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
References
HLA Class I and Genetic Susceptibility to Type 1 Diabetes: Results from the Type 1 Diabetes Genetics Consortium. Noble JA, et al. Diabetes, 2010 Aug 26. PMID 20798335. Effect of HLA Class I and Class II Alleles on Progression from Autoantibody Positivity to Overt Type 1 Diabetes in Children with Risk-Associated Class II Genotypes. Lipponen K, et al. Diabetes, 2010 Aug 25. PMID 20739684. Infectious mononucleosis-linked HLA class I single nucleotide polymorphism is associated with multiple sclerosis. Jafari N, et al. Mult Scler, 2010 Aug 24. PMID 20736246. [Associations of Human Leukocyte Antigen-A, B, DRB1 Genes with Leukemia Patients in Anhui Province of China.] Liao YQ, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2010 Jul. PMID 20723328. Longer survival associated with HLA-A*03, B*14 among 212 hemochromatosis probands with HFE C282Y homozygosity and HLA-A and -B typing and haplotyping. Barton JC, et al. Eur J Haematol, 2010 Aug 14. PMID 20722701.
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