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ZEB1 antibody ( Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - ZEB1 antibody ( Ascites) AM1878A
    ZEB1 Antibody (Ascites) (Cat. #AM1878a) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the ZEB1 antibody detected the ZEB1 protein (arrow).
    detail
  • FC - ZEB1 antibody ( Ascites) AM1878A
    ZEB1 Antibody ( Ascites) (Cat. #AM1878a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P37275
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone/Animal Names 213CT14.1.5
Calculated MW 124074 Da
Antigen Region 69-97 aa
Additional Information
Gene ID 6935
Other Names Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8
Target/Specificity This ZEB1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 69-97 amino acids from human ZEB1.
Dilution WB~~1:500~16000
FC~~1:10~50
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZEB1 antibody ( Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZEB1 (HGNC:11642)
Function Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Cellular Location Nucleus
Tissue Location Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas
Research Areas
Citations ( 0 )

Background

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

References

Drake, J.M., et al. J. Biol. Chem. 285(44):33940-33948(2010) Takeyama, Y., et al. Cancer Lett. 296(2):216-224(2010) Nakahata, S., et al. Oncogene 29(29):4157-4169(2010) Mehta, J.S., et al. Invest. Ophthalmol. Vis. Sci. 49(1):184-188(2008) Manavella, P.A., et al. Biochem. Biophys. Res. Commun. 360(3):621-626(2007)

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$ 182.50
Cat# AM1878A
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