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ZEB1 antibody ( Ascites)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, E |
|---|---|
| Primary Accession | P37275 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,K |
| Clone/Animal Names | 213CT14.1.5 |
| Calculated MW | 124074 Da |
| Antigen Region | 69-97 aa |
| Gene ID | 6935 |
|---|---|
| Other Names | Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8 |
| Target/Specificity | This ZEB1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 69-97 amino acids from human ZEB1. |
| Dilution | FC~~1:10~50 WB~~1:500~16000 E~~Use at an assay dependent concentration. |
| Format | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ZEB1 antibody ( Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZEB1 (HGNC:11642) |
|---|---|
| Function | Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity). |
| Cellular Location | Nucleus |
| Tissue Location | Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
References
Drake, J.M., et al. J. Biol. Chem. 285(44):33940-33948(2010) Takeyama, Y., et al. Cancer Lett. 296(2):216-224(2010) Nakahata, S., et al. Oncogene 29(29):4157-4169(2010) Mehta, J.S., et al. Invest. Ophthalmol. Vis. Sci. 49(1):184-188(2008) Manavella, P.A., et al. Biochem. Biophys. Res. Commun. 360(3):621-626(2007)
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