KANK1 Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q14678 |
Reactivity | Human |
Host | Mouse |
Clonality | monoclonal |
Isotype | IgG1,k |
Clone/Animal Names | 1773CT634.49.61 |
Calculated MW | 147289 Da |
Gene ID | 23189 |
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Other Names | KN motif and ankyrin repeat domain-containing protein 1, Ankyrin repeat domain-containing protein 15, Kidney ankyrin repeat-containing protein, KANK1, ANKRD15, KANK, KIAA0172 |
Target/Specificity | This KANK1 antibody is generated from a mouse immunized with a recombinant protein of human KANK1. |
Dilution | WB~~1:2000 IHC-P~~1:25 |
Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | KANK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KANK1 |
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Synonyms | ANKRD15, KANK, KIAA0172 |
Function | Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration (PubMed:25961457). Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competitive interaction with 14-3-3 adapter proteins to sequester them from active complexes (PubMed:25961457). Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competitive interaction with BAIAP2 to block its association with activated RAC1 (PubMed:25961457). Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma. Regulates Rac signaling pathways (PubMed:25961457). |
Cellular Location | Cell projection, ruffle membrane. Cytoplasm. Nucleus Note=Colocalizes with KIF21A in membrane ruffles (PubMed:19559006) Shuttles between the cytoplasm and nucleus (PubMed:16968744) [Isoform 2]: Cytoplasm. Nucleus Note=Shuttles between the cytoplasm and nucleus |
Tissue Location | Widely expressed. Isoform 1 is predominantly expressed in heart and kidney. Isoform 2 probably is widely expressed at basic levels. |
Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma.
References
Nagase T.,et al.DNA Res. 3:17-24(1996).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Sarkar S.,et al.J. Biol. Chem. 277:36585-36591(2002).
Wang Y.,et al.Biochem. Biophys. Res. Commun. 330:1247-1253(2005).
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