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Voltage Gated Potassium Channel, Kv2.2 Subunit Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Voltage Gated Potassium Channel, Kv2.2 Subunit Antibody AN1063
    Western blot of rat brain homogenate showing specific immunolabeling of the ~125k voltage-gated potassium channel, Kv2.2 (Control). The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide block).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63099
Reactivity Rat
Host Rabbit
Clonality polyclonal
Calculated MW 100 KDa
Additional Information
Gene ID 621349
Gene Name KCNB2
Other Names Potassium voltage-gated channel subfamily B member 2, CDRK, Voltage-gated potassium channel subunit Kv22, Kcnb2
Target/Specificity Synthetic peptide corresponding to amino acid residues specific to the Kv2.2 subunit conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.
Antibody Specificity Specific for the ~125k voltage gated potassium channel, Kv 2.2 subunit.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVoltage Gated Potassium Channel, Kv2.2 Subunit Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).


Blaine JT, Ribera AB (1998) Heteromultimeric potassium channels formed by members of the Kv2 subfamily. J Neurosci 18:9585-9593.
Burger C, Ribera AB (1996) Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development. J Neurosci 16:1412-1421.
Hwang PM, Fotuhi M, Bredt DS, Cunningham AM, Snyder SH (1993) Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily. J Neurosci 13:1569-1576.
Michaelevski I, Chikvashvili D, Tsuk S, Singer-Lahat D, Kang YH, Linial M, Gaisano HY, Fili O, Lotan I (2003) Direct interaction of target SNAREs with the Kv2.1 channel - Modal regulation of channel activation and inactivation gating. J Biol Chem 278:34320-34330.
Pongs O (1999) Voltage-gated potassium channels: from hyperexcitability to excitement. FEBS Lett 452:31-35.
Yan L, Figueroa DJ, Austin CP, Liu Y, Bugianesi RM, Slaughter RS, Kaczorowski GJ, Kohler MG (2004) Expression of voltage-gated potassium channels in human and rhesus pancreatic islets. Diabetes 53:597-607.
D. Guan, T. Tkatch, D. J. Surmeier, W. E. Armstrong, and R. C. Foehring (2007) Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons J. Physiol., Jun 2007; 581: 941 - 960.

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$ 365.00
Cat# AN1063
Availability: 7-10 days
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