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Phospho-Ser129 Alpha Synuclein Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P37377 |
| Reactivity | Rat |
| Predicted | Bovine, Human, Mouse, Monkey |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 15 KDa |
| Gene ID | 29219 |
|---|---|
| Gene Name | SNCA |
| Other Names | Alpha-synuclein, Snca |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~15 kDa alpha-synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser129 Alpha Synuclein Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).
References
Okochi M, Walter J, Koyama A, Nakajo S, Baba M, Iwatsubo T, Meijer L, Kahle PJ, Haass C (2000) Constitutive phosphorylation of the Parkinson’s disease associated alpha-synuclein. J Biol Chem Jan 7;275(1): 390-7.
Neumann M, Kahle PJ, Giasson BI, Ozmen L, Borroni E, Spooren W, Muller V, Odoy S, Fujiwara H, Hasegawa M, Iwatsubo T, Trojanowski JQ, Kretzschmar HA, Haass C (2002) Misfolded proteinase k resistant hyperphosphorylated alpha-synuclein in aged transgenic mice with locomotor deterioration and in human alpha synucleinopathies. J Clin Invest Nov; 110(10):1403-5.
Smith WW, Margolis RL, Li X, Troncoso JC, Lee MK, Dawson VL, Dawson TM, Iwatsubo T, Ross CA (2005) Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci June 8; 25(23):5544-5552.
Masliah E, Rockenstein E, Adame A, Alford M, Crews L, Hashimoto M, Suebert P, Lee M, Goldstein J, Chilcote T, Games D, Schenk D (2005) Effects of alpha-synuclein immunization in a mouse model of Parkinson’s disease. Neuron Jun 16;46(6):857-868.
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