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Myelin Basic Protein (MBP) Antibody

Chicken polyclonal antibody

     
  • WB - Myelin Basic Protein (MBP) Antibody AN1200
    Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 18& 22k MBP protein.
    detail
  • IF - Myelin Basic Protein (MBP) Antibody AN1200
    Rat mixed neuron/glial cultures stained with chicken anti-MyelinBasic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stainsan oligodendrocyte and some membrane shed from this cell. Other cells in the field includeneurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P02688
Reactivity Bovine, Human, Mouse, Rat
Host Chicken
Clonality polyclonal
Calculated MW 18/22 KDa
Additional Information
Gene ID 24547
Gene Name MBP
Other Names Myelin basic protein, MBP, Mbp
Target/Specificity Three peptide sequences conserved in higher vertebrate MBP proteins.
Dilution WB~~ 1:5000
IF~~ 1:500
Format Total IgY fraction.
Antibody Specificity Specific for the ~18k & 22k myelin basic protein in Western blots of ratbrain homogenate
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyelin Basic Protein (MBP) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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citation

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Background

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa.

References

Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P. (1971) Basic A1 protein of the myelin membrane: the complete amino acid sequence. J. Biol. Chem. 246:5770-5784.
Marty MC, Alliot F, Rutin J, Fritz R, Trisler D. and Pessac B. (2002) The myelin basic protein gene is expressed in differentiated blood cell lineages and in hematopoietic progenitors. Proc. Nat. Acad. Sci. 99:8856-8861.

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$ 310.00
Cat# AN1200
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