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Phospho-Ser150 Troponin I (cardiac) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser150 Troponin I (cardiac) Antibody AN1227
    Western blot of mouse heart lysate showing specific immunolabeling of ~25k cTnI protein phosphorylated at Ser150 (control). Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48787
Reactivity Mouse, Rat
Predicted Human, Monkey
Host Rabbit
Clonality polyclonal
Calculated MW 25 KDa
Additional Information
Gene ID 21954
Gene Name TNNI3
Other Names Troponin I, cardiac muscle, Cardiac troponin I, Tnni3
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Antibody Specificity Specific for the ~25k cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser150 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
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Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).


Raquel Sancho Solis, Ying Ge, Jeffery W. Wlaker. (2011) A Preferred AMPK phosphorylation site adjacent to the inhibitory loop of cardiac and skeletal Troponin I.
Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.

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$ 365.00
Cat# AN1227
Availability: 7-10 days
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