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Phospho-Ser150 Troponin I (cardiac) Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P48787 |
| Reactivity | Mouse, Rat |
| Predicted | Human, Monkey |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 25 KDa |
| Gene ID | 21954 |
|---|---|
| Gene Name | TNNI3 |
| Other Names | Troponin I, cardiac muscle, Cardiac troponin I, Tnni3 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. |
| Antibody Specificity | Specific for the ~25k cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser150 Troponin I (cardiac) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
References
Raquel Sancho Solis, Ying Ge, Jeffery W. Wlaker. (2011) A Preferred AMPK phosphorylation site adjacent to the inhibitory loop of cardiac and skeletal Troponin I.
Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.
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