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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ANGPTL1 Antibody (N-term)   

ANGPTL1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 Antibody (N-term) (Cat. #AP10105a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ANGPTL1 antibody detected the ANGPTL1 protein (arrow).
  • IHC-P - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 antibody (N-term) (Cat. #AP10105a) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the ANGPTL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 Antibody (N-term) (Cat. #AP10105a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O95841
Other Accession NP_004664.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 12-44 aa
Additional Information
Other Names Angiopoietin-related protein 1, Angiopoietin-3, ANG-3, Angiopoietin-like protein 1, ANGPTL1, ANG3, ANGPT3, ARP1
Target/Specificity This ANGPTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-44 amino acids from the N-terminal region of human ANGPTL1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANGPTL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANGPTL1
Synonyms ANG3, ANGPT3, ARP1
Cellular Location Secreted.
Tissue Location Highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine. Weakly expressed in testis, ovary, colon, pancreas, kidney and stomach EMBL; AF107253; AAD19608.1; -; mRNA EMBL; AY358278; AAQ88645.1; -; mRNA EMBL; AB056476; BAB40691.1; -; mRNA EMBL; AL355520; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC050640; AAH50640.1; -; mRNA CCDS; CCDS1327.1; - RefSeq; NP_004664.1; NM_004673.3 RefSeq; XP_005245634.1; XM_005245577.2 UniGene; Hs.591474; - ProteinModelPortal; O95841; - SMR; O95841; - STRING; 9606.ENSP00000234816; - iPTMnet; O95841; - PhosphoSitePlus; O95841; - BioMuta; ANGPTL1; - EPD; O95841; - jPOST; O95841; - PaxDb; O95841; - PeptideAtlas; O95841; - PRIDE; O95841; - ProteomicsDB; 51085; - Ensembl; ENST00000234816; ENSP00000234816; ENSG00000116194 Ensembl; ENST00000367629; ENSP00000356601; ENSG00000116194 GeneID; 9068; - KEGG; hsa:9068; - UCSC; uc001gma.4; human CTD; 9068; - DisGeNET; 9068; - EuPathDB; HostDB:ENSG00000116194.12; - GeneCards; ANGPTL1; - HGNC; HGNC:489; ANGPTL1 HPA; HPA009976; - MIM; 603874; gene neXtProt; NX_O95841; - OpenTargets; ENSG00000116194; - PharmGKB; PA24794; - eggNOG; KOG2579; Eukaryota eggNOG; ENOG410ZYS4; LUCA GeneTree; ENSGT00940000155091; - HOGENOM; HOG000037129; - HOVERGEN; HBG103336; - InParanoid; O95841; - OMA; FKDCQHA; - OrthoDB; 525245at2759; - PhylomeDB; O95841; - TreeFam; TF336658; - SignaLink; O95841; - SIGNOR; O95841; - GeneWiki; ANGPTL1; - GenomeRNAi; 9068; - PRO; PR:O95841; - Proteomes; UP000005640; Chromosome 1 Bgee; ENSG00000116194; Expressed in 168 organ(s), highest expression level in lower esophagus ExpressionAtlas; O95841; baseline and differential Genevisible; O95841; HS GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0005615; C:extracellular space; IDA:MGI GO; GO:0005102; F:signaling receptor binding; TAS:ProtInc GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IEA:Ensembl CDD; cd00087; FReD; 1 InterPro; IPR036056; Fibrinogen-like_C InterPro; IPR002181; Fibrinogen_a/b/g_C_dom InterPro; IPR020837; Fibrinogen_CS Pfam; PF00147; Fibrinogen_C; 1 SMART; SM00186; FBG; 1 SUPFAM; SSF56496; SSF56496; 1 PROSITE; PS00514; FIBRINOGEN_C_1; 1 PROSITE; PS51406; FIBRINOGEN_C_2; 1 2: Evidence at transcript level; Coiled coil; Complete proteome; Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal SIGNAL 1 23 CHAIN 24 491 Angiopoietin-related protein 1 /FTId=PRO_0000009118 DOMAIN 271 491 Fibrinogen C-terminal {ECO:0000255|PROSITE-ProRule:PRU00739} COILED 80 168 CARBOHYD 160 160 N-linked (GlcNAc...) asparagine CARBOHYD 188 188 N-linked (GlcNAc...) asparagine DISULFID 280 309 {ECO:0000255|PROSITE-ProRule:PRU00739} DISULFID 432 445 {ECO:0000255|PROSITE-ProRule:PRU00739} SEQUENCE 491 AA; 56720 MW; 3C4DB8DEF6CF7E99 CRC64; MKTFTWTLGV LFFLLVDTGH CRGGQFKIKK INQRRYPRAT DGKEEAKKCA YTFLVPEQRI TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID VLQLVVDVDG NIVNEVKLLR KESRNMNSRV TQLYMQLLHE IIRKRDNSLE LSQLENKILN VTTEMLKMAT RYRELEVKYA SLTDLVNNQS VMITLLEEQC LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ RDPGYPRDLM PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW LGLENIYMLS NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG TYQGNAGDSM MWHNGKQFTT LDRDKDMYAG NCAHFHKGGW WYNACAHSNL NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL RAVQMMIKPI D
Research Areas
Citations (0)

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Background

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro.

References

Di Carlo, C., et al. Fertil. Steril. 91(6):2315-2323(2009)
Post, S., et al. J. Vasc. Res. 45(3):244-250(2008)
Dhanabal, M., et al. Biochem. Biophys. Res. Commun. 333(2):308-315(2005)
Smagur, A., et al. Acta Biochim. Pol. 52(4):875-879(2005)
Lee, H.J., et al. FASEB J. 18(11):1200-1208(2004)

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Discontinued
Cat# AP10105a
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