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ANGPTL1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 Antibody (N-term) (Cat. #AP10105a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ANGPTL1 antibody detected the ANGPTL1 protein (arrow).
    detail
  • IHC-P - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 antibody (N-term) (Cat. #AP10105a) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the ANGPTL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ANGPTL1 Antibody (N-term) AP10105a
    ANGPTL1 Antibody (N-term) (Cat. #AP10105a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession O95841
Other Accession NP_004664.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56720 Da
Antigen Region 12-44 aa
Additional Information
Gene ID 9068
Other Names Angiopoietin-related protein 1, Angiopoietin-3, ANG-3, Angiopoietin-like protein 1, ANGPTL1, ANG3, ANGPT3, ARP1
Target/Specificity This ANGPTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-44 amino acids from the N-terminal region of human ANGPTL1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANGPTL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANGPTL1
Synonyms ANG3, ANGPT3, ARP1
Cellular Location Secreted.
Tissue Location Highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine. Weakly expressed in testis, ovary, colon, pancreas, kidney and stomach
Research Areas
Citations (0)
citation

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Background

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro.

References

Di Carlo, C., et al. Fertil. Steril. 91(6):2315-2323(2009)
Post, S., et al. J. Vasc. Res. 45(3):244-250(2008)
Dhanabal, M., et al. Biochem. Biophys. Res. Commun. 333(2):308-315(2005)
Smagur, A., et al. Acta Biochim. Pol. 52(4):875-879(2005)
Lee, H.J., et al. FASEB J. 18(11):1200-1208(2004)

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Discontinued
Cat# AP10105a
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