FBXW12 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q6X9E4 |
Other Accession | NP_001153399.1, NP_001153401.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 53056 Da |
Antigen Region | 291-320 aa |
Gene ID | 285231 |
---|---|
Other Names | F-box/WD repeat-containing protein 12, F-box and WD-40 domain-containing protein 12, F-box only protein 35, FBXW12, FBW12, FBXO12, FBXO35 |
Target/Specificity | This FBXW12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the C-terminal region of human FBXW12. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | FBXW12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FBXW12 |
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Synonyms | FBW12, FBXO12, FBXO35 |
Function | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex (PubMed:26171402). Promotes degradation of interleukin-22 receptor subunit IL22RA1 in resting and IL22-stimulated conditions by facilitating its ubiquitination (PubMed:26171402). Functions as a cell growth suppressor (PubMed:26171402). |
Tissue Location | Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
References
Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)
Zeng, L., et al. Mol. Biol. Rep. 31(1):51-57(2004)
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