MAP1D Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q6UB28 |
Other Accession | NP_954697.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37088 Da |
Antigen Region | 306-334 aa |
Gene ID | 254042 |
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Other Names | Methionine aminopeptidase 1D, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03174}, MAP 1D {ECO:0000255|HAMAP-Rule:MF_03174}, MetAP 1D {ECO:0000255|HAMAP-Rule:MF_03174}, 341118 {ECO:0000255|HAMAP-Rule:MF_03174}, Methionyl aminopeptidase type 1D, mitochondrial, Peptidase M 1D {ECO:0000255|HAMAP-Rule:MF_03174}, METAP1D, MAP1D |
Target/Specificity | This MAP1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the N-terminal region of human MAP1D. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MAP1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | METAP1D |
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Synonyms | MAP1D |
Function | Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis. |
Cellular Location | Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03174, ECO:0000269|PubMed:14532271} |
Tissue Location | Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Leszczyniecka, M., et al. Oncogene 25(24):3471-3478(2006)
Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003)
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