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MAP1D Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAP1D Polyclonal Antibody AP57207
    Sample:
    SW480(Human) Cell Lysate at 30 ug
    Primary: Anti-MAP1D (bs-18659R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 35 kD
    Observed band size: 35 kD
    detail
  • IHC-P - MAP1D Polyclonal Antibody AP57207
    Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (bs-18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q6UB28
Reactivity Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 37088 Da
Additional Information
Gene ID 254042
Other Names Methionine aminopeptidase 1D, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03174}, MAP 1D {ECO:0000255|HAMAP-Rule:MF_03174}, MetAP 1D {ECO:0000255|HAMAP-Rule:MF_03174}, 3.4.11.18 {ECO:0000255|HAMAP-Rule:MF_03174}, Methionyl aminopeptidase type 1D, mitochondrial, Peptidase M 1D {ECO:0000255|HAMAP-Rule:MF_03174}, METAP1D, MAP1D
Dilution WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name METAP1D
Synonyms MAP1D
Function Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.
Cellular Location Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03174, ECO:0000269|PubMed:14532271}
Tissue Location Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level)
Research Areas
Citations (0)
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$ 350.00
Cat# AP57207
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