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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   DLX1 Antibody (Center)   

DLX1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - DLX1 Antibody (Center) AP22281c
    Overlay histogram showing HeLa cells stained with AP22281c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22281c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - DLX1 Antibody (Center) AP22281c
    Anti-DLX1 Antibody (Center) at 1:2000 dilution + Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P56177
Other Accession Q64317
Reactivity Human, Mouse
Predicted Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 27320 Da
Additional Information
Gene ID 1745
Other Names Homeobox protein DLX-1, DLX1
Target/Specificity This DLX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 84-118 amino acids from the Central region of human DLX1.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDLX1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DLX1
Function Plays a role as a transcriptional activator or repressor (PubMed:14671321). Inhibits several cytokine signaling pathways, such as TGFB1, activin-A/INHBA and BMP4 by interfering with the transcriptional stimulatory activity of transcription factors, such as MSX2, FAST2, SMAD2 and SMAD3 during hematopoietic cell differentiation (PubMed:14671321). Plays a role in terminal differentiation of interneurons, such as amacrine and bipolar cells in the developing retina (By similarity). Likely to play a regulatory role in the development of the ventral forebrain (By similarity). May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in hematopoietic cell lines.
Research Areas
Citations (0)

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Background

Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions (By similarity).

References

Chiba S.,et al.Proc. Natl. Acad. Sci. U.S.A. 100:15577-15582(2003).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Simeone A.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:2250-2254(1994).

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$ 325.00
$ 109.00
Cat# AP22281c
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