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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CKS2 Antibody (N-Term)   

CKS2 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - CKS2 Antibody (N-Term) AP22318a
    Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - CKS2 Antibody (N-Term) AP22318a
    All lanes : Anti-CKS2 Antibody (N-Term) at 1:2000 dilution Lane 1: Caco2 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P33552
Other Accession Q2KJI1, P56390
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 9860 Da
Additional Information
Gene ID 1164
Other Names Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2
Target/Specificity This CKS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the human region of human CKS2.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCKS2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CKS2
Function Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Research Areas
Citations (0)

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Background

Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.

References

Richardson H.E.,et al.Genes Dev. 4:1332-1344(1990).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 325.00
$ 109.00
Cat# AP22318a
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