|Application ||IHC-P, WB, E|
|Other Accession||P32189, Q0IID9, Q4R4D5, Q14409|
|Calculated MW||60594 Da|
|Antigen Region||487-515 aa|
|Other Names||Glycerol kinase 2, GK 2, Glycerokinase 2, ATP:glycerol 3-phosphotransferase 2, Glycerol kinase, testis specific 2, GK2, GKP2, GKTA|
|Target/Specificity||This Glycerol kinase (GPK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human Glycerol kinase (GPK2).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Glycerol kinase (GPK2) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Key enzyme in the regulation of glycerol uptake and metabolism.|
|Cellular Location||Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=In sperm the majority of the enzyme is bound to mitochondria.|
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Provided below are standard protocols that you may find useful for product applications.
The human glycerol kinase gene family consists of at least 3 expressed loci. The GK1 locus on Xp21.3 is the site of mutations (deletions) causing glycerol kinase deficiency. It comprises 19 exons and is probably ancestral to several other genes which, because they are intronless, are suspected of having arisen by reverse transcriptase mediated events. These include 2 genes on chromosome 4. They are expressed as a single mRNA species in testis where expression is at a high level.
Sargent, C.A., et al., Hum. Mol. Genet. 3(8):1317-1324 (1994).
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