- CITATIONS: 1
|Application ||IHC-P, WB, E|
|Other Accession||O08561, Q8BKC8, O02810, Q5VWB9|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||91379 Da|
|Antigen Region||20-51 aa|
|Other Names||Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KB, PIK4CB|
|Target/Specificity||This PI4KCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-51 amino acids from the N-terminal region of human PI4KCB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PI4KCB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).|
|Cellular Location||Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus. Golgi apparatus membrane. Cytoplasm, perinuclear region Note=Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex Recruited to the Golgi apparatus membrane by ACBD3, GGA2 is also involved in the recruitment.|
|Tissue Location||Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.|
Provided below are standard protocols that you may find useful for product applications.
Phosphoinositides are pivotal precursors to important second messengers and as signaling and molecules. Phosphatidylinositol 4-kinases (PI4Ks) are are crucial regulators of the phosphoinsitide cascade. PI4KCB is a wortmannin-sensitive PI 4-kinase responsible for regulating the synthesis of agonist-sensitive pools of polyphosphoinositides. The cellular reservoir of PI4KCB is predominantly cytosolic, however the protein is is activated strongly by recruitment to the membrane to stimulate phosphatidylinositol 4,5-bisphosphate synthesis at the plasma membrane. PI4KCB contains an N-terminal lipid kinase unique domain, which is shared by members of both the PI3 kinase and PI4 kinase families, and a C-terminal catalytic domain, which defines this protein as a member of a much larger protein/lipid kinase family.
Wei, Y.J., et al., J. Biol. Chem. 277(48):46586-46593 (2002).
Balla, A., et al., J. Biol. Chem. 277(22):20041-20050 (2002).
Sorensen, S.D., et al., Mol. Pharmacol. 53(5):827-836 (1998).
Saito, T., et al., DNA Res. 4(4):301-305 (1997).
Suzuki, K., et al., DNA Res. 4(4):273-280 (1997).
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