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NIP7 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NIP7 Antibody (C-term) AP8691b
    Western blot analysis of NIP7 (arrow) using rabbit polyclonal NIP7 Antibody (C-term) (Cat. #AP8691b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NIP7 gene.
    detail
  • WB - NIP7 Antibody (C-term) AP8691b
    Anti-NIP7 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - NIP7 Antibody (C-term) AP8691b
    NIP7 Antibody (C-term) (Cat. #AP8691b) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9Y221
Other Accession Q56P27, Q9CXK8
Reactivity Human
Predicted Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20463 Da
Antigen Region 150-177 aa
Additional Information
Gene ID 51388
Other Names 60S ribosome subunit biogenesis protein NIP7 homolog, KD93, NIP7
Target/Specificity This NIP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-177 amino acids from the C-terminal region of human NIP7.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNIP7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NIP7
Function Required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly.
Cellular Location Nucleus, nucleolus
Tissue Location Expressed in hematopoietic stem/progenitor cells.
Research Areas
Citations (0)
citation

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Background

Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.

References

Liu,J.F., et.al., J. Struct. Biol. 148 (3), 370-374 (2004)
Chagnon,P., et.al., Am. J. Hum. Genet. 71 (6), 1443-1449 (2002)

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$ 182.50
$ 70.00
Cat# AP8691b
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