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Cpn10 Antibody

  • IHC - Cpn10 Antibody ASM10452
    Immunohistochemistry analysis using Rabbit Anti-Cpn10 Polyclonal Antibody (ASM10452). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (ASM10452) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Strong basal layer punctate staining in the cytoplasm, weakens as it ascends to upper epidermis..
  • WB - Cpn10 Antibody ASM10452
    Western blot analysis of Rat brain cell lysates showing detection of Cpn10 protein using Rabbit Anti-Cpn10 Polyclonal Antibody (ASM10452). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Cpn10 Polyclonal Antibody (ASM10452) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61604
Other Accession NP_002148.1
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Pig, Bovine, Xenopus, Dog, Sheep, Guinea Pig
Clonality Polyclonal
Description Rabbit Anti-Human Cpn10 Polyclonal
Target/Specificity Detects ~ 10kDa.
Other Names 10kDa Chaperonin Antibody, Chaperonin 10 Antibody, Cpn 10 Antibody, EPF Antibody, GROES Antibody, Heat Shock 10kD protein1 Antibody, HSP10 Antibody, HSPE1 Antibody
Immunogen Human Cpn10 peptide AA 91-101
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.3, 0.02% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-193 was sufficient for detection of Cpn10 in 10 µg of Hela lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion | Mitochondrion Matrix
Research Areas
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Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kDa (HSP10s). Cpn10 acts as a co-chaperone and interacts with the HSP60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).


1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549.
2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460.
3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560.
4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.

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Cat# ASM10452
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