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Anti-Cpn10 Antibody

     
  • ICC - Anti-Cpn10 Antibody ABO11098
    Anti-Cpn10 antibody, ABO11098, ICCICC: A549 Cell
    detail
  • WB - Anti-Cpn10 Antibody ABO11098
    Anti-Cpn10 antibody, ABO11098, Western blottingAll lanes: Anti Cpn10 (ABO11098) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Rat Testis Tissue Lysate at 50ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 11KDObserved bind size: 11KD
    detail
  • IHC - Anti-Cpn10 Antibody ABO11098
    Anti-Cpn10 antibody, ABO11098, IHC(P)IHC(P): Human Intestinal Cancer Tissue
    detail
  • IHC - Anti-Cpn10 Antibody ABO11098
    Anti-Cpn10 antibody, ABO11098, IHC(P)IHC(P): Mouse Intestine Tissue
    detail
  • IHC - Anti-Cpn10 Antibody ABO11098
    Anti-Cpn10 antibody, ABO11098, IHC(P)IHC(P): Rat Intestine Tissue
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P61604
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial(HSPE1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 3336
Other Names 10 kDa heat shock protein, mitochondrial, Hsp10, 10 kDa chaperonin, Chaperonin 10, CPN10, Early-pregnancy factor, EPF, HSPE1
Calculated MW 10932 MW KDa
Application Details Immunocytochemistry , 0.5-1 µg/ml, Human, -
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse
Subcellular Localization Mitochondrion matrix.
Protein Name 10 kDa heat shock protein, mitochondrial(Hsp10)
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name HSPE1
Function Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:7912672, PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)
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Background

HSPE1(heat shock 10kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

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$ 240.00
Cat# ABO11098
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