Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Mitochondrion   >   TRAP1 Antibody   

TRAP1 Antibody

     
  • ICC/IF - TRAP1 Antibody ASM10469
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - TRAP1 Antibody ASM10469
    Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~75 kDa TRAP1 protein using Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 12 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa.
  • ICC/IF - TRAP1 Antibody ASM10469
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TRAP1 Polyclonal Antibody (ASM10469) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q12931
Other Accession NP_057376.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human TRAP1 Polyclonal
Target/Specificity Detects ~75 kDa, multiple isoforms.
Other Names Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody
Immunogen Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-209 was sufficient for detection of Trap-1 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

The 90 kDa heat shock protein (HSP90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to beassociated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).

References

1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312.
2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46.
3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

Cat# ASM10469
Availability: Inquire
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900
Cedarlane Labs
+1 (800) 721-1644
+1 (336) 513-5138

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"