|Application ||WB, ICC/IF|
|Description||Rabbit Anti-Human ATG5 Polyclonal|
|Target/Specificity||Predicted molecular weight at ~32.4kDa. Observed molecular weights at ~48-60kDa based on ATG12-ATG5 heterodimer.|
|Other Names||APG5-like Antibody, ASP Antibody, hAPG5 Antibody|
|Immunogen||Synthetic peptide from the mid-protein of human ATG5|
|Purification||Peptide Affinity Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||A 1:1000 dilution of SPC-611 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|
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Provided below are standard protocols that you may find useful for product applications.
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).
1. Pyo J.O., et al. (2005) J Biol Chem. 280(21): 20722-20729.
2. Mizushima N., et al. (2003) Mol Biol Cell. 15(3): 1101-1111.
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