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Calcium Sensing Receptor Antibody

     
  • ICC/IF - Calcium Sensing Receptor Antibody ASM10566
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (ASM10566). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (ASM10566) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.
  • WB - Calcium Sensing Receptor Antibody ASM10566
    Western blot analysis of Rat Kidney cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (ASM10566). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (ASM10566) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 130kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P41180
Other Accession NP_000379.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human Calcium Sensing Receptor Polyclonal
Target/Specificity Detects ~140 kDa.
Other Names Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism Antibody, HHC Antibody, Extracellular calcium sensing receptor Antibody, FIH Antibody, Parathyroid Cell calcium-sensing receptor Antibody, CASR Antibody, CASR_HUMAN Antibody, Ca sensing receptor Antibody, Extracellular calcium-sensing receptor Antibody, PCAR1 Antibody, FHH Antibody, Ca2+ sensing receptor Antibody, EIG8 Antibody, Hypocalciuric hypercalcemia 1 Antibody, PCaR1 Antibody, GPRC2A Antibody, Extracellular calcium sensing receptor [Precursor] Antibody, PCAR 1 Antibody, Parathyroid cell calcium-sensing receptor 1 Antibody, HHC1 Antibody, Parathyroid Cell calcium sensing receptor Antibody, CAR Antibody, MGC138441 Antibody, NSHPT Antibody, CaSR Antibody, Ca2+ sensing receptor 1 Antibody, Parathyroid Ca(2+) sensing receptor 1 Antibody,
Immunogen Synthetic peptide of Human calcium-sensing receptor (CASR)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-713 was sufficient for detection of calcium-sensing receptor (CASR) in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Multi-Pass Membrane Protein
Research Areas
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Cat# ASM10566
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