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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ARF5 Antibody (monoclonal) (M01)   

ARF5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ARF5.

  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in HeLa ( (Cat # AT1176a )
  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in PC-12((Cat # AT1176a )
  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    ARF5 monoclonal antibody (M01), clone 1B4. Western Blot analysis of ARF5 expression in Raw 264.7((Cat # AT1176a )
  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    ARF5 monoclonal antibody (M01), clone 1B4 Western Blot analysis of ARF5 expression in A-431 ( (Cat # AT1176a )
  • WB - ARF5 Antibody (monoclonal) (M01) AT1176a
    Western Blot analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.

    Lane 1: ARF5 transfected lysate(20.5 KDa).
    Lane 2: Non-transfected lysate.
  • IF - ARF5 Antibody (monoclonal) (M01) AT1176a
    Immunofluorescence of monoclonal antibody to ARF5 on HeLa cell. [antibody concentration 15 ug/ml]
  • E - ARF5 Antibody (monoclonal) (M01) AT1176a
    Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P84085
Other Accession BC003043
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1B4
Calculated MW 20530 Da
Additional Information
Gene ID 381
Other Names ADP-ribosylation factor 5, ARF5
Target/Specificity ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsARF5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.


1.A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.Reiling JH, Olive AJ, Sanyal S, Carette JE, Brummelkamp TR, Ploegh HL, Starnbach MN, Sabatini DMNat Cell Biol. 2013 Dec;15(12):1473-85. doi: 10.1038/ncb2865. Epub 2013 Nov 3.2.ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HWMol Biol Cell. 2013 Aug;24(16):2570-81. doi: 10.1091/mbc.E13-04-0197. Epub 2013 Jun 19.3.GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.Takashima K, Saitoh A, Hirose S, Nakai W, Kondo Y, Takasu Y, Kakeya H, Shin HW, Nakayama K.Cell Struct Funct. 2011;36(2):223-35.4.Class II ADP-Ribosylation factors are required for efficient secretion of dengue viruses.Kudelko M, Brault JB, Kwok K, Li MY, Pardigon N, Peiris JS, Bruzzone R, Despres P, Nal B, Wang PG.J Biol Chem. 2011 Nov 21.5.Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity.Puxeddu E, Uhart M, Li CC, Ahmad F, Pacheco-Rodriguez G, Manganiello VC, Moss J, Vaughan M.Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6158-63. Epub 2009 Mar 30.6.EFA6 facilitates the assembly of the tight junction by coordinating an Arf6-dependent and independent pathway.Klein S, Partisani M, Franco M, Luton F.J Biol Chem. 2008 Oct 31;283(44):30129-38. Epub 2008 Sep 8.7.The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles.Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ.J Biol Chem. 2007 Mar 30;282(13):9591-9. Epub 2007 Feb 2.

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$ 350.00
Cat# AT1176a
Availability: 7-10 days
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