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ARF5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P84085 |
| Other Accession | P84083, P84084, NP_001653.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20530 Da |
| Antigen Region | 80-108 aa |
| Gene ID | 381 |
|---|---|
| Other Names | ADP-ribosylation factor 5, ARF5 |
| Target/Specificity | This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARF5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARF5 |
|---|---|
| Function | GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. |
| Cellular Location | Golgi apparatus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P84084}; Lipid-anchor {ECO:0000250|UniProtKB:P84084} |
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Provided below are standard protocols that you may find useful for product applications.
Background
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].
References
Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Kondo, A., et al. Mol. Biol. Cell 11(4):1315-1327(2000)
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