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CCT5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CCT5.

     
  • IF - CCT5 Antibody (monoclonal) (M01) AT1428a
    Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - CCT5 Antibody (monoclonal) (M01) AT1428a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (85.25 KDa) .
    detail
  • WB - CCT5 Antibody (monoclonal) (M01) AT1428a
    CCT5 monoclonal antibody (M01), clone 4E5-4B1 Western Blot analysis of CCT5 expression in Hela ( (Cat # AT1428a )
    detail
  • IHC - CCT5 Antibody (monoclonal) (M01) AT1428a
    Immunoperoxidase of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]
    detail
  • E - CCT5 Antibody (monoclonal) (M01) AT1428a
    Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P48643
Other Accession BC006543
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4E5-4B1
Calculated MW 59671 Da
Additional Information
Gene ID 22948
Other Names T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, CCT5, CCTE, KIAA0098
Target/Specificity CCT5 (AAH06543, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCCT5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified.

References

1.Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.Donadio E, Giusti L, Cetani F, Da Valle Y, Ciregia F, Giannaccini G, Pardi E, Saponaro F, Torregrossa L, Basolo F, Marcocci C, Lucacchini A.Proteome Sci. 2011 Jun 8;9(1):29.2.A proteomic approach to study parathyroid glands.Giusti L, Cetani F, Ciregia F, Da Valle Y, Donadio E, Giannaccini G, Banti C, Pardi E, Saponaro F, Basolo F, Berti P, Miccoli P, Pinchera A, Marcocci C, Lucacchini A.Mol Biosyst. 2011 Mar;7(3):687-99. Epub 2010 Dec 21.3.Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T.Virology. 2010 Nov 17. [Epub ahead of print]

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$ 350.00
Cat# AT1428a
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