CCT5 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant CCT5.
|Application ||WB, IHC, IF, E|
|Calculated MW||59671 Da|
|Other Names||T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, CCT5, CCTE, KIAA0098|
|Target/Specificity||CCT5 (AAH06543, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||CCT5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified.
1.Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.Donadio E, Giusti L, Cetani F, Da Valle Y, Ciregia F, Giannaccini G, Pardi E, Saponaro F, Torregrossa L, Basolo F, Marcocci C, Lucacchini A.Proteome Sci. 2011 Jun 8;9(1):29.2.A proteomic approach to study parathyroid glands.Giusti L, Cetani F, Ciregia F, Da Valle Y, Donadio E, Giannaccini G, Banti C, Pardi E, Saponaro F, Basolo F, Berti P, Miccoli P, Pinchera A, Marcocci C, Lucacchini A.Mol Biosyst. 2011 Mar;7(3):687-99. Epub 2010 Dec 21.3.Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T.Virology. 2010 Nov 17. [Epub ahead of print]
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