|Other Names||LIM and calponin homology domains-containing protein 1, LIMCH1, KIAA1102|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13141b was selected from the C-term region of LIMCH1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.|
|Cellular Location||Cytoplasm, cytoskeleton, stress fiber|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
LIMCH1 contains one CH (calponin-homology) domain and one LIM zinc-binding domain. There are nine named isoforms.
Rose, J. Phd, et al. Mol. Med. (2010) In press :Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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