|Other Names||Serine protease HTRA1, 3421-, High-temperature requirement A serine peptidase 1, L56, Serine protease 11, HTRA1, HTRA, PRSS11|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1331b was selected from the C-term region of human HtrA1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.|
|Cellular Location||Cell membrane. Secreted. Cytoplasm, cytosol Note=Predominantly secreted (PubMed:15208355). Also found associated with the plasma membrane (PubMed:21297635)|
|Tissue Location||Widely expressed, with strongest expression in placenta (at protein level). Secreted by synovial fibroblasts. Up- regulated in osteoarthritis and rheumatoid arthritis synovial fluids and cartilage as compared with non-arthritic (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
HtrA1 is a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.
Howes, N., et al., Clin Gastroenterol Hepatol 2(3):252-261 (2004).Chien, J., et al., Oncogene 23(8):1636-1644 (2004).Hu, S.I., et al., J. Biol. Chem. 273(51):34406-34412 (1998).Zumbrunn, J., et al., Genomics 45(2):461-462 (1997).Zumbrunn, J., et al., FEBS Lett. 398 (2-3), 187-192 (1996).
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