MAPKAP1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9BPZ7 |
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Clone Names | 90904147 |
Gene ID | 79109 |
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Other Names | Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1, MAPKAP1, MIP1, SIN1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP8641b was selected from the C-term region of human MAPKAP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | MAPKAP1 |
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Synonyms | MIP1, SIN1 |
Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. |
Cellular Location | Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus |
Tissue Location | Ubiquitously expressed, with highest levels in heart and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MAPKAP1 is highly similar to the yeast SIN1 protein, a stress-activated protein kinase.
References
Jin,J., et.al., Curr. Biol. 14 (16), 1436-1450 (2004)Sarbassov,D.D., et.al., Science 307 (5712), 1098-1101 (2005)
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