|Calculated MW||55391 Da|
|Other Names||Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog|
|Application Notes||Reconstitute to 1 unit per µl in PBS containing 15% glycerol.|
|Storage||-70°C; Lyophilized powder|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA or Ac-IETD-AFC.
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Muzio M.,et al.Cell 85:817-827(1996).
Fernandes-Alnemri T.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996).
Srinivasula S.M.,et al.J. Biol. Chem. 272:18542-18545(1997).
Grenet J.,et al.Gene 226:225-232(1999).
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