Caspase-8, human recombinant protein
Caspase-8
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q14790 |
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Calculated MW | 55391 Da |
Gene ID | 841 |
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Gene Symbol | CASP8 |
Other Names | Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-8 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Lyophilized powder |
Storage | -70°C; Lyophilized powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA or Ac-IETD-AFC.
References
Boldin M.P.,et al.Cell 85:803-815(1996).
Muzio M.,et al.Cell 85:817-827(1996).
Fernandes-Alnemri T.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996).
Srinivasula S.M.,et al.J. Biol. Chem. 272:18542-18545(1997).
Grenet J.,et al.Gene 226:225-232(1999).
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