Anti-Human FGF-10 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND

Application
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Goat |
| Clonality | Polyclonal |
| Preparation | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFGF-10. Anti-Human FGF-10 specific antibody was purified by affinity chromatography employing immobilized hFGF-10 matrix. |
|---|---|
| WesternBlot | To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect hFGF-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human FGF-10 (60-139BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-10. |
| Immunohistochemistry | This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 µg/ml - 2.5 µg/ml with an overnight iincubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human FGF-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |

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