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> home > Products > Primary Antibodies > Antibody Collections > Antibody Pairs > Anti-Human IL-5 Antibody
Anti-Human IL-5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Preparation | Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-5. Anti-Human IL-5 specific antibody was purified by affinity chromatography employing immobilized Human IL-5 matrix. |
|---|---|
| WesternBlot | To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Sandwich | To detect Human IL-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-5 (60-005BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-5. |
| Immunohistochemistry | This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. |
| Neutralization | To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-5 (5.0 ng/ml), a concentration of 0.05 - 0.15 µg/ml of this antibody is required. |
| Formulation | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Storage | -20°C |
| Precautions | Anti-Human IL-5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Research Areas
Citations (0)
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 275.00
$ 216.00
Cat# ABG10286-U100
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